SINGLE-CELL SPATIAL BIOLOGY

Focus: In 2017, our lab began leveraging single-cell spatial biology techniques to uncover the dynamics of cell state heterogeneity and how states of tumor and stromal cells influence solid tumor progression. Toward this end, we are taking an unbiased approach to profile the primary and metastatic microenvironments of solid tumors. To date, we have 64-plex protein, 1K-plex RNA and 6K-plex RNA datasets collected for human pancreas and breast microarrays that represent a spectrum of disease and patient characteristics using the Bruker NanoString CosMx platform. Currently, we offer analyses for breast and pancreas tissues, but additional CosMx data from colon, skin, liver and prostate TMAs will be available soon.

Relevant publication: Hamalian et al. 2021 Oncogene 

Bioinformatics Collaborators: Dr. Mary Lauren Benton (Baylor)

Potential Collaborations: We welcome new collaborations that can use these data to support additional lines of investigation. If interested, please do the following...

1. Review the characteristics of our breast (BRM961b) and pancreas (PA722) TMAs analyzed to date. CosMx datasets will be available soon for prostate (BC19021a), melanoma (ME1921), liver (LV1221a) and colon (CO702e) TMAs. See below for details.

2. Review the protein (64-plex) or RNA (1K-plex or 6K-plex) panels below to identify proteins/genes of interest.

3. Complete our CosMx Data Analysis Request Form below or at https://forms.office.com/r/MzAq189w9t.

4. Email jonathan_kelber@baylor.edu with additional questions.